Overview

These instructions document the wet lab protocols for the lab's core sequencing workflows, from DNA extraction through running the MiniSeq. The pages in this section walk you through each stage of the workflow:

• DNA Extraction: Manual extraction with the PowerSoil Pro kit or automated extraction with the epMotion.
• PCR Amplification: Primary and indexing (q)PCR for FoodSeq (trnL / 12SV5) amplicons.
• Gel Electrophoresis: Running agarose gels or E-Gels to verify amplification.
• Amplicon Cleaning: Bead cleaning with AMPure XP beads.
• Quantification and Pooling: Equimolar pooling and running the pool on a bioanalyzer.
• Sequencing: 1.2 pM pool, ~30% PhiX spike-in.